immortalized human neuronal cells
I am currently looking for human immortalized neuronal cells. literatures I've come across thus far are on immortalized neuronal progenitor, stem cells. May I have some recommendation?
View Articlelabeling parvalbumin interneurons in culture
Anyone have a good protocol for this? I have the sigma antibody for parvalbumin (http://www.sigmaaldrich.com/catalog/product/sigma/p3088?lang=en®ion=US), which has been published to work quite...
View Articleclampfit downloading
Hi electrophysiologists if you are in this forum, I have troubles to analyze my cells within clampfit 10.2, in the computer of the lab. I am not very familiar with. but I learned how to use...
View Articlepost-mortem BBB permeability marker
Hi Can anyone suggest a reliable marker for blood brain barrier permeability in fresh frozen cryostat sectioned tissue? Can I just post-fix and immunostain for albumin, or will I get artifact from...
View ArticleMembrane sensitive dyes: The DIBAC family
Dear all, I am currently working on membrane potential sensitive dyes and I am really struggling with DIBAC4(3). This is the protocol that I have followed by reading the literature. I am using HepG2...
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Mice LTP is not working
Dear friend I am Sudarshan Patil, started to work on mice LTP. Trying to measure fEPSP in vivo. I am getting very good/typical signal from the mice dentate gyrus (Please see attached photo). I tried...
View ArticleSham or Vehicle?
Hey guys, This is my first post here, hope I can get some help. We are collaborating with a research of parkinson disease rat model; and in this model we inject a toxic substance in a certain...
View Articledouble immunofluorescence
we want to see the phosphorylation of kinase in in interneurons. by using kinase antibody and the marker of interneurons. the signal of interneurons marker is a bit low. while when we tried kinase...
View Articlemeasure of cell size
Hi all, I want to measure the size of cells from slice, which are labeled with a specific antibody by immunofluorescence. I took pictures of the slices, and I open them in photoshop, to count the...
View ArticlePerfusion of neonatal mouse?
Hi, has anyone done an intracardiac perfusion on an infant mouse? I keep reading papers that just say to do it, but I am not sure how- what gauge needle would I use, what flow rate, how to get the...
View ArticleQuestions on mind uploading
I'm new here as you guessed and I'm doing research on mind uploading and I had 3 questions 1. Using the copy and transfer method what would your kind be uploaded to ? A super computer desktop ? A...
View Articlemouse stellate/cervicothoracic ganglion dissection
Hi, Can anyone tell me how to dissect out the stellate/cervicothoracic ganglion from an adult mouse? Thank you
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Neurophysiology- NERVE online laboratory
Dear experts, I want to complete the laboratory experiments of "NERVE" from the University of Chicagi and I am having several misunderstandings I will be glad to receive help with: 1. As far as I...
View Articlelentivirus production/infectivity problems
Hi everyone, I sent a lentiviral plasmid out to a company to be packaged. My insert size is very large, 5'LTR to 3'LTR size is 10.2Kb. I got a titer of 5x10^8 estimated by qPCR of the WPRE element....
View Articlecomercial BBB kit
Hello all, Does anyone know if a comercial kit exists to make a blood brain barrier, containing the endothelial cells and the transwell? Thanks! BioPe
View ArticleImmunocytochemistry neurons problems with Triton X 100
good evening, I am working on neurons. Today I was wrong with the dilution of Triton X100 to permeabilize the cells. I should prepare 0.1% Triton X 100 but I realized that I prepared 0.8% Triton X...
View ArticleMeasuring IPSPs in a pacemaker neuron
Hi guys, I am trying to measure IPSPs in a cell using current clamp. The cell does not have a stable membrane potential, but rather an oscillating membrane potential, because it is a pacemaker...
View Articleneed help with nerves and receptors MC question
Could anyone help me and tell me the answers to: For each of the following physiological responses, select the most appropriate type of nerve and the receptor in the tissue that mediates the...
View ArticleWhy GFP/YFP in neurons is lost at cytometer after mouse brain dissociation?
Hi everyone! I'm trying to isolate parvalbumin-positive neurons of hippocampus and cortex in transgenic mice with YFP expressed only in parvalbumin neurons (Cre/Loxp method). I want to extract RNA from...
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ELISA dopamine
Hi guys, I want to start a ELISA assay to measurement released dopamine of SH-SY5Y cells after a stimulation with an agonist of nicotinic receptor. I don´t know the best way to preserve...
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